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As a reference a mixed standard fatty acid concentration was injected. The mixed standard of fatty acids contained acetate, propionate, (iso- and n-) butyrate and (iso- and n-) valerate with a concentration of 50 mM for each acid. The fatty acid concentration in the samples should be less than 5 mM. Analysis conditions were described by Gallert and Winter (1997). 3 Biogas Composition The biogas produced during anaerobic digestion of whey was measured with a gasometer by water displacement (Fa. Ritter or Braud, Bochum-Langendreer, Germany).

The well mixed samples were evaporated in a weighed vessel and dried to constant weight. For determining the total solid (TS), the sample was evaporated at 103 to 105oC. The organic fraction of TS (OTS or volatile solids) was obtained by subtraction of the mineral content (residual ash after oxidation at 550 + 50oC) from the total solids content. 9 Microscopical Examination The microscopically examination was used for counting the bacterial cell number, observing the shape of bacterial cells and flocs, and also distinguishing the presence of methanogenic bacteria.

35 l CH4 per g COD removed. The specific methane production, which was close to the theoretical yield of methane per 1 g of COD that was degraded was reached at an OLR lower than 10 g COD·l-1·d-1. Increasing the organic loading up to 35 g COD·l-1·d-1 reduced the specific methane production to les then 40% of the theoretically expected amount. The decreasing of specific methane production had the same tendency as the results of COD removal (Figure 16). When the intermediately excreted fatty acids were converted to methane, the concentration of residual organic compounds in the effluent was low and the percentage of COD removal was high.

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Album Panini Calciatori Mondiali Messico


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